To introduce surface plasmons and localized plasmons. To present plasmon excitation techniques with application to SPR biochips and antibody/antigen kinetics.
Course outline
Historical background. Optics of metals. Drude model. Intraband/interband transition. Validity of Drude model. Brendel-Bormann model. Metal interfaces. Evanescent waves. Surface plasmons. Excitation/Resonance. Otto experiment. Kretschmann-Raether experiment. Other excitation techniques. Labelless biochips. SPR biochips. Surface functionalization. Langmuir model. Antibody/antigen kinetics. Sensograms. Biochip performances. Electrospotting. SPRi.
Practical work
Practical works (4 hours): Numerical simulation and study of a time-resolved SPR with a FEM software (COMSOL)
Project (12 hours): design and construction of an SPR chip microfluidic cavity, characterization and observation of kinetic antibodies / antigens by optical imaging.
Acquired skills
To understand principles of surface plasmon and localized plasmon resonances.
To be able to use and design SPR biochips under specifications compatible with health IT.